Composite
Part:BBa_K3739037
Designed by: Ziyan Han Group: iGEM21_XMU-China (2021-09-09)
J23100-B0030-Aly01-his-hutH-B0010
Aly01 is a signal peptide from the alginate lyase of V.natriegens and GFP can show the secretion effciency of Aly01
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 232
Illegal NgoMIV site found at 668
Illegal NgoMIV site found at 1403
Illegal NgoMIV site found at 1639
Illegal AgeI site found at 305
Illegal AgeI site found at 1132
Illegal AgeI site found at 1628 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |