Part:BBa_K4035006

Dimerization of the copper metallothionein 1 : CUP1-(EAAAK)4-CUP1

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. In order to improve the copper fixation efficiency in yeast, CUP1 was linked to a second sequence of the same protein. The linker is a rigid linker and is made of four successive (EAAAK) amino acid sequences.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 408
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 408
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 408
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 408
  
• 1000
  COMPATIBLE WITH RFC[1000]