Composite

Part:BBa_K3739039

Designed by: Ziyan Han   Group: iGEM21_XMU-China   (2021-09-09)
Revision as of 20:45, 9 September 2021 by Registry (Talk | contribs)

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J23100-B0030-LMT-his-CBM-hutH-B0010

LMT is a signal peptide from the lytic murein transglycosylase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 661
    Illegal NgoMIV site found at 1097
    Illegal NgoMIV site found at 1832
    Illegal NgoMIV site found at 2068
    Illegal AgeI site found at 407
    Illegal AgeI site found at 497
    Illegal AgeI site found at 734
    Illegal AgeI site found at 1561
    Illegal AgeI site found at 2057
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None