Coding

Part:BBa_K3739011

Designed by: Han Ziyan   Group: iGEM21_XMU-China   (2021-09-08)
Revision as of 10:30, 8 September 2021 by Registry (Talk | contribs)

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LMT-his-CBM-hutH

LMT is a signal peptide from the lytic murein transglycosylase of V.natriegens, his-tag enable us to purify the linking protein, CBM can bind to cellulose and hutH is a histidine transaminase which converts histidine into urocanic acid


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 597
    Illegal NgoMIV site found at 1033
    Illegal NgoMIV site found at 1768
    Illegal NgoMIV site found at 2004
    Illegal AgeI site found at 343
    Illegal AgeI site found at 433
    Illegal AgeI site found at 670
    Illegal AgeI site found at 1497
    Illegal AgeI site found at 1993
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None