Composite

Part:BBa_K3796008

Designed by: Li Mingyang   Group: iGEM21_CAU_China   (2021-09-04)
Revision as of 17:04, 4 September 2021 by Registry (Talk | contribs)

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capABC for gamma-PGA synthesis

This composite part contains three coding sequences, capA,capB,capC, which are joined upstream and downstream of the promoter P0864+RBS and T7 terminator, respectively. It is used for the synthesis and extracellular transport of gamma-polyglutamic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2046
    Illegal XbaI site found at 2341
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2046
    Illegal NheI site found at 2487
    Illegal NheI site found at 2491
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2046
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2046
    Illegal XbaI site found at 2341
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2046
    Illegal XbaI site found at 2341
    Illegal PstI site found at 423
    Illegal PstI site found at 1691
    Illegal PstI site found at 2322
    Illegal NgoMIV site found at 2679
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None