Composite
Part:BBa_K3796008
Designed by: Li Mingyang Group: iGEM21_CAU_China (2021-09-04)
capABC for gamma-PGA synthesis
This composite part contains three coding sequences, capA,capB,capC, which are joined upstream and downstream of the promoter P0864+RBS and T7 terminator, respectively. It is used for the synthesis and extracellular transport of gamma-polyglutamic acid.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2046
Illegal XbaI site found at 2341
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2046
Illegal NheI site found at 2487
Illegal NheI site found at 2491
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2046
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2046
Illegal XbaI site found at 2341
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2046
Illegal XbaI site found at 2341
Illegal PstI site found at 423
Illegal PstI site found at 1691
Illegal PstI site found at 2322
Illegal NgoMIV site found at 2679 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |