Coding

Part:BBa_K4035001:Design

Designed by: Anissa Hammi   Group: iGEM21_EPFL   (2021-08-30)
Revision as of 16:01, 30 August 2021 by Registry (Talk | contribs)

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CUP1 fused to Aga2 and tagged with a V5 epitope


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 319
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 376
    Illegal PstI site found at 319
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 562
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 319
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The start and stop codon of the CUP1 genomic sequence were removed in order to fuse the protein to Aga2 and V5.


Source

The CUP1 (BBa_M45090) sequence is the genomic sequence of the copper metallotionein 1 protein and was inserted in the pCTcon2_V5 plasmid that was already containing Aga2, V5 tag and the Gal1 promoter.

References