Part:BBa_K4035001

CUP1 fused to Aga2 and tagged with a V5 epitope

Copper metallotionein CUP1 (BBa_M45090) is a protein responsible for copper binding protein in the yeast Saccharomyces cerevisiae. Normally expressed intracellularly, CUP1 was fused to the A-agglutinin-binding subunit Aga2 (BBa_K416000) in order to be displayed on the outter yeast cell surface. This fusion protein also contained a V5 tag in order to check its expression by Western Blot and Immunostaining. The expression was under the control of the Gal1 promoter, so that the protein was expressed only in the presence of galactose.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 319
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 376
  Illegal PstI site found at 319
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 562
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 319
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 319
  
• 1000
  COMPATIBLE WITH RFC[1000]