Part:BBa_K3838999
J-TES4
Butyric acid promotes the repair of intestinal epithelial cells, strengthens the intestinal mucosal barrier, and can inhibit multiple inflammatory pathways. Butyric acid is also the main energy source of colon epithelial mucosa. The proportion of patients with intestinal butyric acid bacteria decreased, and butyric acid can not be taken directly by mouth, only enema, etc., more painful. Engineered microorganisms enable sustained, long-term drug delivery. Solution: We use the fatty acid synthesis pathway of lactic acid bacteria and Escherichia coli and introduce exogenous TES4 gene can achieve the production of butyric acid with glucose as substrate. Tes4 enzyme has some specificity for four-carbon fatty acids. When the fatty acid is synthesized to 4-carbon, the enzyme will release it from ACP to obtain butyric acid. In our team project,the related part is composed as follows: J23100 promoter+RBS+tes4+6*his tag+T7Te terminator+rrnB T1 terminator.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 322
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 322 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 351
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 322
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 322
- 1000COMPATIBLE WITH RFC[1000]
| None |