Composite

Part:BBa_K3838222

Designed by: Siyang Yu   Group: iGEM21_SZU-China   (2021-08-30)
Revision as of 07:13, 30 August 2021 by SiyangYu-0901 (Talk | contribs)


J-BSH

Bilesalthydrolase (BSH), responsible for hydrolysis in combination with bile salts to produce free bile acids and amino acids. BSH activity in gastrointestinal tract was mainly from Firmicutes (30%), Bacteroidetes (14.4%) and actinobacteria (8.9%). Impaired microbial enzyme activity in IBD patients leads to impaired bile acid metabolism, characterized by the inability to hydrolyze, transform, and desulphurize bile salts. Microbial BSH activity removes glycine or taurine molecular coupling to produce unbound bile acids. Abnormal intestinal bile salt metabolism enhances the inflammatory response of the intestinal epithelium, which worsens IBD. It has been documented that the ingestion of probiotics with BSH activity can promote the bioconversion of intestinal bile salts, thereby alleviating the inflammatory response induced by high doses of intestinal bile salts. Therefore, we selected heterologous expression of BSH gene from Lactobacillus acidophilus. In a team project, the BSH-related parts are structured as follows: J23100 promoter +RBS+BSH+T7 terminator

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 969
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None