Part:BBa_K3981004
sTRAIL
Tumor necrosis factor (TNF) - related apoptosis inducing ligand (TRAIL), originally identified as a member of the TNF family, can induce apoptosis in various cancer cells. TRAIL mediated induction of apoptosis in most transformed tumour cells occurs through stimulation of their cognate receptors DR4 (or TRAIL1) and DR5 (or TRAIL2). TRAIL is a type transmembrane protein that can be produced in a soluble form by protease mediated cleavage of its extracellular region or by bacterial expression of this recombinant form containing its extracellular region. Structural studies of TRAIL reveal that it forms a trimer, which further binds to the trimeric protein DR5. TRAIL can form, in its native soluble form, trimers that induce apoptosis. As a type II transmembrane protein, traces can be cleaved by specific proteases to form a soluble molecule in the extracellular region. The study of protein crystal structure shows that soluble TRAIL (sTRAIL) forms a homotrimer, which is the key structure of receptor recognition and apoptosis. We improved the sTRAIL sequence, deleted several amino acids and optimized the codon to make it easier to express in E. coli Nissle 1917 (ECN). By connecting an isoleucine zipper at the N end of sTRAIL, it leads to its trimerization and has higher cytotoxic activity than its natural state.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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