Plasmid_Backbone
Part:BBa_K3728002
Designed by: Eric Shih Group: iGEM21_Mingdao (2021-08-19)
pTol2: a Tol2 vector - the Tol2 transposable element
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Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3452
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 29
Illegal NotI site found at 3458 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3452
Illegal BglII site found at 3340
Illegal XhoI site found at 3419 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3452
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3452
Plasmid lacks a suffix.
Illegal XbaI site found at 3467
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 642 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 1818
Illegal SapI.rc site found at 2900
[edit]
Categories
Parameters
None |