Plasmid_Backbone

Part:BBa_K3728002

Designed by: Eric Shih   Group: iGEM21_Mingdao   (2021-08-19)
Revision as of 04:07, 24 August 2021 by F91445122 (Talk | contribs)


pTol2: a Tol2 vector - the Tol2 transposable element

T--Mingdao--BBa K3728002-1.png



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3452
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 29
    Illegal NotI site found at 3458
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 3452
    Illegal BglII site found at 3340
    Illegal XhoI site found at 3419
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 3452
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 3452
    Plasmid lacks a suffix.
    Illegal XbaI site found at 3467
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 642
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 1818
    Illegal SapI.rc site found at 2900


[edit]
Categories
Parameters
None