Composite

Part:BBa_K3809003

Designed by: Francisco Javier Castañeda Villagran   Group: iGEM21_TecCEM   (2021-08-13)
Revision as of 17:38, 13 August 2021 by Registry (Talk | contribs)

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katE regulated by BBa_J23100

This Biobrick contains a katE gene with an RBS (BBa_B0030), spacer (BBa_B0040), terminator (BBa_B0010) and promoter (BBa_J23100). It was designed to be expressed as part of a plasmid, to act as a selection cassette for a modified strain of E. coli without genes involved in the methabolic pathways of glycerol and peroxide. It may be also used for other purposes not stated here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1962
    Illegal BamHI site found at 1872
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None