Part:BBa_K3838777
SHSP
SHSP is a stress protein, also known as heat shock protein, which is a group of proteins preferentially synthesized by cells. Compared with the mutant strains without the plasmid encoding SHSP, the parental strains with the plasmid encoding SHSP were significantly more resistant to temperature changes from 42℃ to 62℃, and there was a significant difference in cell survival rate between the SHSP plasmid carrying streptococcus thermophilus strain and the SHSP elimination plasmid under heat shock condition. The cloned SHSP gene was transformed into streptococcus thermophilus strains lacking plasmid encoding SHSP, resulting in increased resistance at 60℃ or pH 3.5, and increased growth ability at 52℃. Meanwhile, the presence of PET-SHSP and the overexpression of SHSP gene demonstrated its ability to protect E. coli cells from ethanol stress. Results from the literature showed that escherichia coli cells overexpressing SHSP gene of the smaller plasmid pQC2 of Streptococcus thermophilus ST-QC significantly increased their resistance to heat, acid and ethanol stress. Therefore, we chose HSP as a non-antibiotic e. coli screening method.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 79
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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