Coding

Part:BBa_K3971004

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-09)
Revision as of 17:43, 9 August 2021 by Registry (Talk | contribs)

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sps - Sucrose-phosphate synthase gene in Cyanobacteria.

The sps gene is native to the Synechococcus sp, and the sps catalyzed reaction is the rate limiting step in sucrose biosynthesis in cyanobacteria [1]. It catalyzes the rate-limiting conversion of uridine diphosphate glucose (UDP-Glu) and fructose 6-phosphate (F6P) into sucrose 6-phosphate (S6P) in the sucrose synthesis pathway. In Synechococcus elongatus 2973, the sps enzyme has both sucrose-phosphate synthase and sucrose-phosphate phosphatase activities [2].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1676
    Illegal PstI site found at 1714
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1676
    Illegal NheI site found at 1869
    Illegal PstI site found at 1714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1676
    Illegal XhoI site found at 1453
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1676
    Illegal PstI site found at 1714
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1676
    Illegal PstI site found at 1714
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None