Regulatory

Part:BBa_K3971000:Design

Designed by: Misaal Bedi   Group: iGEM21_IISER-Pune-India   (2021-08-01)
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Strong constitutive cyanobacterial PcpcB-7942 mutant promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used the sequence as is, since this promoter was created from Synechococcus elongatus PCC 7942 which is a close relative of Synechococcus elongatus UTEX 2973 (1) and their genomes are 99.8% identical (2), thus we used the sequence as is. The sequence did not contain any restriction sites for enzymes we used during cloning as well.

References:

1: Yu, Jingjie, et al. "Synechococcus elongatus UTEX 2973, a fast growing cyanobacterial chassis for biosynthesis using light and CO 2." Scientific reports 5.1 (2015): 1-10.

2:Cui, Jinyu, et al. "Improved salt tolerance and metabolomics analysis of Synechococcus elongatus UTEX 2973 by overexpressing Mrp Antiporters." Frontiers in bioengineering and biotechnology 8 (2020): 500.


Source

This promoter was generously provided to us by The Wangikar Lab at the Indian Institute of Technology, Bombay.

References