Coding

Part:BBa_K3941002:Design

Designed by: Begüm Esra Aytan   Group: iGEM21_Saint_Joseph   (2021-07-26)
Revision as of 11:43, 1 August 2021 by Begumaytan (Talk | contribs)


EGII (C99V)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is created based on only the coding sequence of Trichoderma reesei endoglucanase II gene by excluding introns and non-coding exon sites. Signal sequence is also removed. Codon optimization was applied. A single aminoacid mutation has been studied as the Cystein aminoacid in 99 aminoacid position is changed with Valine aminoacid.


Source

The source of this pars is Trichoderma reesei endoglucanase II gene.

References