Part:BBa_K1486000

CpxR coding region

CpxR is a transciption factor that dimerizes and becomes active when phosphorylated by the transmembrane histidine kinase CpxA. CpxA becomes active when the bacterial cell is submitted to different kinds of shock (pH, temperature, pressure, protein misfolding in the inter-membrane space...).

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

• Group: City of London UK 2021
• Author: Julian Chen
• Summary: Added information collated from existing scientific studies

It is a response regulator of the regulatory system 'CpxA/CpxR', which is a 2 component regulatory system. It responds to the envelope stress response with the activation of the downstream genes including cpxP, degP, dsbA and ppiA. It is required for efficient binding of stationary phase cells to hydrophobic surfaces, part of the process of biofilm formation and when induced upon cell surface binding, it induces the genes it controls. “Surface Sensing and Adhesion of Escherichia Coli Controlled by the Cpx-Signaling Pathway.” n.d. Www.uniprot.org. Accessed July 30, 2021. https://www.uniprot.org/citations/11830644. It also binds and activates transcription from the degP promoter-the binding is enhanced by phosphorylation. This system combats a variety of extracytoplasmic protein-mediated toxicities by increasing the transcription of the periplasmic protease, DegP in concert with sigma factor E, as well as that of CpxP protein. Other downstream effectors may include SrkA.

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 61
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 472
  
• 1000
  COMPATIBLE WITH RFC[1000]