Part:BBa_K116601

HtlB (ftsH) coding region from E. coli

The HtlB (ftsH) coding region from E. coli. It can be used to degrade many different proteins.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

• Group: City of London UK 2021
• Author: Julian Chen
• Summary: Added information collated from existing scientific studies

Plays a role in the quality control of integral membrane proteins. Degrades membrane proteins in a processive manner starting at either the N- or C-terminus; recognition requires a cytoplasmic tail of about 20 residues with no apparent sequence requirements.

It degrades ceratin membrane proteins that have not been assembled into complexes such as SecY, F0 ATPase subunit a and YccA, as well as cytoplasmic proteins sigma-32, LpxC, KdtA and phage lambda cII protein Degrades C-terminal-tagged cytoplasmic proteins which are tagged with an 11-amino-acid nonpolar destabilizing tail via a mechanism involving the 10SA (SsrA) stable RNA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 219
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 586
  Illegal AgeI site found at 1459
  
• 1000
  COMPATIBLE WITH RFC[1000]