Coding

Part:BBa_K116601

Designed by: Jesse Wu   Group: iGEM08_NYMU-Taipei   (2008-10-29)
Revision as of 19:53, 28 July 2021 by JYC (Talk | contribs)


HtlB (ftsH) coding region from E. coli

The HtlB (ftsH) coding region from E. coli. It can be used to degrade many different proteins.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

Group: City of London UK 2021 Author: Julian Chen Summary: Added information collated from existing scientific studies 

Plays a role in the quality control of integral membrane proteins.

Degrades membrane proteins in a processive manner starting at either the N- or C-terminus; recognition requires a cytoplasmic tail of about 20 residues with no apparent sequence requirements. Degrades C-terminal-tagged cytoplasmic proteins which are tagged with an 11-amino-acid nonpolar destabilizing tail via a mechanism involving the 10SA (SsrA) stable RNA.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 219
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 586
    Illegal AgeI site found at 1459
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme/protease
Parameters
None