Part:BBa_J07006

malE

malE - a monomer. This monomeric protein is used as a negative control when testing for dimerization.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

Group: City of London UK 2021
Author: Lucas Ng
Summary: Added information collated from existing scientific studies

It is the solute-binding protein member of the ABC transporter complex MalEFGK involved in cell chemotaxis via binding to and actively importing maltodextrins ^[1].

Kinetics ^[2]
Maltodextrin	K_M (µM)
Maltose	0.9
Maltriose	2

Its native operator region is induced by maltose and the transcriptional regulator MalT ^[3], while being PNPase-dependently and irreversibly repressed by cold shock ^[4].

References

↑ Davidson, A. L., and H. Nikaido. 1991. “Purification and Characterization of the Membrane-Associated Components of the Maltose Transport System from Escherichia Coli.” The Journal of Biological Chemistry 266 (14): 8946–51. https://pubmed.ncbi.nlm.nih.gov/2026607.
↑ Szmelcman, S., M. Schwartz, T. J. Silhavy, and W. Boos. 1976. “Maltose Transport in Escherichia Coli K12. A Comparison of Transport Kinetics in Wild-Type and Lambda-Resistant Mutants as Measured by Fluorescence Quenching.” European Journal of Biochemistry 65 (1): 13–19. https://doi.org/10.1111/j.1432-1033.1976.tb10383.x.
↑ Kellermann, O., and S. Szmelcman. 1974. “Active Transport of Maltose in Escherichia Coli K12. Involvement of a ‘Periplasmic’ Maltose Binding Protein.” European Journal of Biochemistry 47 (1): 139–49. https://doi.org/10.1111/j.1432-1033.1974.tb03677.x.
↑ Polissi, Alessandra, Walter De Laurentis, Sandro Zangrossi, Federica Briani, Vera Longhi, Graziano Pesole, and Gianni Dehò. 2003. “Changes in Escherichia Coli Transcriptome during Acclimatization at Low Temperature.” Research in Microbiology 154 (8): 573–80. https://doi.org/10.1016/s0923-2508(03)00167-0.

[1] Davidson, A. L., and H. Nikaido. 1991. “Purification and Characterization of the Membrane-Associated Components of the Maltose Transport System from Escherichia Coli.” The Journal of Biological Chemistry 266 (14): 8946–51. https://pubmed.ncbi.nlm.nih.gov/2026607.

[2] Szmelcman, S., M. Schwartz, T. J. Silhavy, and W. Boos. 1976. “Maltose Transport in Escherichia Coli K12. A Comparison of Transport Kinetics in Wild-Type and Lambda-Resistant Mutants as Measured by Fluorescence Quenching.” European Journal of Biochemistry 65 (1): 13–19. https://doi.org/10.1111/j.1432-1033.1976.tb10383.x.

[3] Kellermann, O., and S. Szmelcman. 1974. “Active Transport of Maltose in Escherichia Coli K12. Involvement of a ‘Periplasmic’ Maltose Binding Protein.” European Journal of Biochemistry 47 (1): 139–49. https://doi.org/10.1111/j.1432-1033.1974.tb03677.x.

[4] Polissi, Alessandra, Walter De Laurentis, Sandro Zangrossi, Federica Briani, Vera Longhi, Graziano Pesole, and Gianni Dehò. 2003. “Changes in Escherichia Coli Transcriptome during Acclimatization at Low Temperature.” Research in Microbiology 154 (8): 573–80. https://doi.org/10.1016/s0923-2508(03)00167-0.

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