Part:BBa_T9150

orotidine 5

Coding region for PyrF gene. PyrF is used in the synthesis of uracil in the same pathway that Ura3 is used in yeast. Can also be used as a conterselectable marker in the presence of 5-Fluoroorotic Acid (5-FOA) and the absence of uracil.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

Group: City of London UK 2021
Author: Lucas Ng
Summary: Added information collated from existing scientific studies

PyrF codes for orotidine 5'-phosphate decarboxylase, which catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP) ^[1]:

Involved in pyrimidine metabolism, PyrF is part of the pathway biosynthesizing UMP from orotate de novo ^[2].

References

↑ “Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 27, 2021. https://www.rhea-db.org/rhea/11596.
↑ Jensen, K. F., J. N. Larsen, L. Schack, and A. Sivertsen. 1984. “Studies on the Structure and Expression of Escherichia Coli PyrC, PyrD, and PyrF Using the Cloned Genes.” European Journal of Biochemistry 140 (2): 343–52. https://doi.org/10.1111/j.1432-1033.1984.tb08107.x.

[1] “Rhea - Annotated Reactions Database.” n.d. Rhea. Accessed July 27, 2021. https://www.rhea-db.org/rhea/11596.

[2] Jensen, K. F., J. N. Larsen, L. Schack, and A. Sivertsen. 1984. “Studies on the Structure and Expression of Escherichia Coli PyrC, PyrD, and PyrF Using the Cloned Genes.” European Journal of Biochemistry 140 (2): 343–52. https://doi.org/10.1111/j.1432-1033.1984.tb08107.x.

[1]

[2]

direction	Forward
ec_num	4.1.1.23
kegg	eco:b1281;
protein	PyrF
swisspro	P08244
tag	None