Part:BBa_K864204

OmpF

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

Group: City of London UK 2021
Author: Lucas Ng
Summary: Added information collated from existing scientific studies

This matrix porin forms cell pores facilitating passive diffusion of small molecules across the outer cell membrane. ^[1]

It forms a mixed heterotrimer with osmoporin OmpC which acts as the outer membrane receptor for the CdiA-EC536 toxin, via polymorphisms in OmpC's extracellular loops 4 and 5 ^[2]. It also acts as a receptor for the bacteriophage T2 and is the probable major receptor for colicin E5.

Its native promoter is regulated by the dual transcriptional regulator OmpR, which activates it under low osmolarity but represses it under high osmolarity ^[3].

References

↑ Duval, Valérie, Hervé Nicoloff, and Stuart B. Levy. 2009. “Combined Inactivation of Lon and YcgE Decreases Multidrug Susceptibility by Reducing the Amount of OmpF Porin in Escherichia Coli.” Antimicrobial Agents and Chemotherapy 53 (11): 4944–48. https://doi.org/10.1128/AAC.00787-09.
↑ Beck, Christina M., Julia L. E. Willett, David A. Cunningham, Jeff J. Kim, David A. Low, and Christopher S. Hayes. 2016. “CdiA Effectors from Uropathogenic Escherichia Coli Use Heterotrimeric Osmoporins as Receptors to Recognize Target Bacteria.” PLoS Pathogens 12 (10): e1005925. https://doi.org/10.1371/journal.ppat.1005925.
↑ Nara, Futoshi, Shin-ichi Matsuyama, Takeshi Mizuno, and Shoji Mizushima. 1986. “Molecular Analysis of Mutant OmpR Genes Exhibiting Different Phenotypes as to Osmoregulation of the OmpF and OmpC Genes of Escherichia Coli.” Molecular and General Genetics MGG 202 (2): 194–99. https://doi.org/10.1007/bf00331636.

[1] Duval, Valérie, Hervé Nicoloff, and Stuart B. Levy. 2009. “Combined Inactivation of Lon and YcgE Decreases Multidrug Susceptibility by Reducing the Amount of OmpF Porin in Escherichia Coli.” Antimicrobial Agents and Chemotherapy 53 (11): 4944–48. https://doi.org/10.1128/AAC.00787-09.

[2] Beck, Christina M., Julia L. E. Willett, David A. Cunningham, Jeff J. Kim, David A. Low, and Christopher S. Hayes. 2016. “CdiA Effectors from Uropathogenic Escherichia Coli Use Heterotrimeric Osmoporins as Receptors to Recognize Target Bacteria.” PLoS Pathogens 12 (10): e1005925. https://doi.org/10.1371/journal.ppat.1005925.

[3] Nara, Futoshi, Shin-ichi Matsuyama, Takeshi Mizuno, and Shoji Mizushima. 1986. “Molecular Analysis of Mutant OmpR Genes Exhibiting Different Phenotypes as to Osmoregulation of the OmpF and OmpC Genes of Escherichia Coli.” Molecular and General Genetics MGG 202 (2): 194–99. https://doi.org/10.1007/bf00331636.

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