Coding

Part:BBa_K1500994

Designed by: Kevin Yang   Group: iGEM14_UChicago   (2014-10-17)
Revision as of 10:57, 26 July 2021 by Ng849 (Talk | contribs) (→‎Information contributed by City of London UK (2021))


Dam

Involved in methylating DNA, overexpression causes 6.4 * 10^-4 /// 6.4 * 10^4 mutation rate increase, mostly catalyzing transversions.

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

  • Group: City of London UK 2021
  • Author: Lucas Ng
  • Summary: Added information collated from existing scientific studies

dam causes DNA methylation within the sequence GATC, preventing cleavage by the MboI restriction endonuclease [1]

It is likely to act in postreplication mismatch repair [2] rather than as a member of a restriction modification system, despite sharing sequence specificity with several type II restriction methylases and endonucleases as part of the N(4)/N(6)-methyltransferase family [3]

Furthermore, it may also be involved in DNA replication [4]

Reaction

a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-homocysteine

[5]


[edit]
Categories
Parameters
None