Generator
phoA
Part:BBa_K3767001
Designed by: Griffin Watson-Boehnisch Group: iGEM21_Queens_Canada (2021-06-08)
Alkaline Phosphatase optimized for E. Coli w/ 40x catalytic activity
Usage and Biology
Alkaline phosphatases are used as reporter enzymes in different assays such as Western Blotting and in situ hybridization[1]. Testing human blood for Alkaline Phosphatase levels is a routine test that can reveal different conditions[2].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
Description
References
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
biology | Escherichia coli |
protein | phoA |