Part:BBa_K3513006
JTi1
Promoter JTi2 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. The reconstructed promoter is more than twice as efficient as the original part BBa_K2976009.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Improved by NFLS 2020
Part improvement by NFLS 2020
Team NFLS improved the efficiency of this part by designing a new part called JTi1 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. This new reconstructed promoter is more than twice as efficient as this original part.
We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter.
The improved part is: <a href="https://parts.igem.org/Part: BBa_K3513006">BBa_K3513006</a>
The standard curve:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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