Part:BBa_K3425060

T7 polymerase regulated Juniper GFP substrate for Qβ replicase

BBa_K3425060 is a T7 polymerase regulated Juniper GFP substrate for Qβ replicase constructed from the weaker T7 RNA polymerase promoter, BBa_K3425035, the Qβ5' recognition sequence of Qβ replicase and RBS, BBa_K3425052, Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the Qβ3' recognition sequence of Qβ replicase with terminator, BBa_K3425053.

QB recognition sites
Refereces
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 550
  Illegal XbaI site found at 83
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 550
  Illegal NheI site found at 859
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 550
  Illegal BglII site found at 86
  Illegal BamHI site found at 759
  Illegal XhoI site found at 90
  Illegal XhoI site found at 890
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 550
  Illegal XbaI site found at 83
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 550
  Illegal XbaI site found at 83
  
• 1000
  COMPATIBLE WITH RFC[1000]