Part:BBa_K3600011

prGLR1-yomScarlet-I-tADH1

This cassette was assembled from 6 part plasmids via Golden Gate assembly using the BsmbBI restriction enzyme. The two connectors (pYTK002 and pYTK072), as well as the terminator (pYTK053) and the Ampicilin resistance/backbone (pYTK095) part plasmids are part of the YTK toolkit developed by Lee et al.¹ and can be ordered online. The promoter part plasmid (BBa_K3600004) and the reporter part plasmid (BBa_K3600010) were synthesized by us.

Usage and Biology

This cassette contains the GLR1 promoter upstream of a reporter gene (mScarlet-i). GLR1 is a stress response gene in s.cerevisiae that is regulated by the YAP1 master regulator². This cassette is then assembled with a part plasmid containing Lys2 homology sequences (BBa_K36000012) in another Golden Gate BsmBI assembly, to give a final integration cassette (BBa_K3600017).

Sequence and Features No part name specified with partinfo tag.

References

[1] Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).

[2] Grant CM, MacIver FH, Dawes IW. Stationary‐phase induction of GLR1 expression is mediated by the yAP‐1 transcriptional regulatory protein in the yeast Saccharomyces cerevisiae. Molecular microbiology. 1996 Nov;22(4):739-46.