Translational_Unit

Part:BBa_K3520013

Designed by: Maria-Ioanna Ioannidou   Group: iGEM20_Athens   (2020-10-25)
Revision as of 02:09, 28 October 2020 by Mar-uli (Talk | contribs) (→‎References)

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bcsA-Bacterial Cellulose Synthase A TU


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



This composite part is the transcriptional unit for BcsA. It was made by Type IIS Assembly. See the design tab for more information.

Parts

Promoter: ompA, a constituent Flavobacterial promoter[1][2].
RBS: A Flavobacteriia-specific RBS[1]
CDS: The bcsA gene.
Terminator: A strong universal double terminator.



References

[1] Chen, S., Kaufman, M., Bagdasarian, M., Bates, A., & Walker, E. (2010). Development of an efficient expression system for Flavobacterium strains. Gene, 458(1-2), 1-10. doi: 10.1016/j.gene.2010.02.006
[2] Chen, S., Bagdasarian, M., Kaufman, M., & Walker, E. (2006). Characterization of Strong Promoters from an Environmental Flavobacterium hibernum Strain by Using a Green Fluorescent Protein-Based Reporter System. Applied And Environmental Microbiology, 73(4), 1089-1100. doi: 10.1128/aem.01577-06

Assembly

Figure 1: Type IIS design with RFC10 primers for TU parts.
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Parameters
None