Composite

Part:BBa_K3589208:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)
Revision as of 01:45, 28 October 2020 by Yanniks (Talk | contribs) (→‎Design Notes)


Level 2 spectinomycin resistance + marLac_3xHA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3728
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3728
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3728
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3728
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3588
    Illegal NgoMIV site found at 4307
    Illegal NgoMIV site found at 4758
    Illegal NgoMIV site found at 5311
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the MoClo standard and codon optimized for C. reinhardtii.

Source

YYYYY

References