Composite

Part:BBa_K3425074

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-20)
Revision as of 01:24, 28 October 2020 by Thomas A (Talk | contribs)

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Expression construct of HSP 16.3 with His tag

BBa K3425074 is a Constitutive expression construct of a his-tagged HSP 16.3 protein and is constructed from the constitutive promoter BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, the his-tag equipped HSP 16.3, BBa_K3425073, as well as the double terminator BBa_B0015.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 240
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None