Coding

Part:BBa_K3589105:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-22)
Revision as of 01:17, 28 October 2020 by Yanniks (Talk | contribs) (→‎Source)

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Mutant laccase from Botrytis aclada for Escherichia coli


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 775
    Illegal PstI site found at 463
    Illegal PstI site found at 550
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 775
    Illegal PstI site found at 463
    Illegal PstI site found at 550
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 775
    Illegal BamHI site found at 920
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 775
    Illegal PstI site found at 463
    Illegal PstI site found at 550
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 775
    Illegal PstI site found at 463
    Illegal PstI site found at 550
    Illegal NgoMIV site found at 612
    Illegal AgeI site found at 136
    Illegal AgeI site found at 976
    Illegal AgeI site found at 1059
    Illegal AgeI site found at 1315
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The natural secretion signal of Botrytis aclada was removed. This part is codon-optimized for Escherichia coli.

Source

This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Escherichia coli.

References