Composite

Part:BBa_K3606077

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 01:10, 28 October 2020 by LaGauche (Talk | contribs)

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P9 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P9 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5547
    Illegal XbaI site found at 5532
    Illegal XbaI site found at 6856
    Illegal SpeI site found at 5960
    Illegal PstI site found at 2370
    Illegal PstI site found at 2403
    Illegal PstI site found at 5946
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5547
    Illegal NheI site found at 6918
    Illegal SpeI site found at 5960
    Illegal PstI site found at 2370
    Illegal PstI site found at 2403
    Illegal PstI site found at 5946
    Illegal NotI site found at 5539
    Illegal NotI site found at 5951
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5547
    Illegal BamHI site found at 3366
    Illegal XhoI site found at 5697
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5547
    Illegal XbaI site found at 5532
    Illegal XbaI site found at 6856
    Illegal SpeI site found at 5960
    Illegal PstI site found at 2370
    Illegal PstI site found at 2403
    Illegal PstI site found at 5946
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5547
    Illegal XbaI site found at 5532
    Illegal XbaI site found at 6856
    Illegal SpeI site found at 5960
    Illegal PstI site found at 2370
    Illegal PstI site found at 2403
    Illegal PstI site found at 5946
    Illegal NgoMIV site found at 2032
    Illegal AgeI site found at 2204
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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