Coding

Part:BBa_K3657002:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-20)
Revision as of 01:07, 28 October 2020 by JuliaLanger (Talk | contribs)

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RNA binding Pumilio Homology Domain (Uracil)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pumilio Domains are specific for the different RNA bases, by varying the amino acids at position 16 and 20. For the uracil binding Pumby Domain, they are asparagine and glutamine, respectively. For more information on the design, visit iGEM Heidelberg 2020

Source

Drosophila melanogaster (Part of the Puf protein)

References

Shuyun Dong and Yang Wang and C. Cassidy-Amstutz and Gang Lu and R. Bigler and Mark R. Jezyk and C. Li and T. Hall and Z. Wang (2011) Specific and Modular Binding Code for Cytosine Recognition in Pumilio/FBF (PUF) RNA-binding Domains*.The Journal of Biological Chemistry 286, 26732 - 26742

Katarzyna P Adamala and D. Martin-Alarcon and E. Boyden (2016) Programmable RNA-binding protein composed of repeats of a single modular unit.Proceedings of the National Academy of Sciences 113, E2579 - E2588