Composite
Part:BBa_K3425062
Designed by: Tereza Hubackova Group: iGEM20_UofUppsala (2020-10-25)
β-subunit of Qβ replicase under control of inducible pCAD promoter
BBa_K3425062 is a β-subunit of Qβ replicase under control of inducible pCAD promoter, and is constructed from the pCadBA operator and promoter, BBa_K3425101, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1496
Illegal XhoI site found at 1004
Illegal XhoI site found at 1170 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |