Composite

Part:BBa_K3425061

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)
Revision as of 00:41, 28 October 2020 by Thomas A (Talk | contribs)


Constitutive expression construct of Qβ replicase

BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.

From observations have can it be stated that this construct tends to mutate in high copy number plasmids

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1377
    Illegal XhoI site found at 885
    Illegal XhoI site found at 1051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None