Composite

Part:BBa_K3425060

Designed by: Tereza Hubackova   Group: iGEM20_UofUppsala   (2020-10-25)
Revision as of 00:36, 28 October 2020 by Thomas A (Talk | contribs)


T7 polymerase regulated Juniper GFP substrate for Qβ replicase

BBa_K3425060 is a T7 polymerase regulated Juniper GFP substrate for Qβ replicase constructed from the weaker T7 RNA polymerase promoter, BBa_K3425035, the Qβ5' recognition sequence of Qβ replicase and RBS, BBa_K3425052, Juniper GFP for Type IIS iGEM standard assembly, BBa_K3425048, as well as the Qβ3' recognition sequence of Qβ replicase with terminator, BBa_K3425053.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 550
    Illegal NheI site found at 859
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 550
    Illegal BglII site found at 86
    Illegal BamHI site found at 759
    Illegal XhoI site found at 90
    Illegal XhoI site found at 890
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 550
    Illegal XbaI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None