Composite

Part:BBa_K3657021:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 00:11, 28 October 2020 by Liza ch (Talk | contribs) (→‎Design Notes)

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RNA Motif GUAGAG targeted PPR - Ribonuclease A Complex


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequences of the PPR proteins were directly concatenated to each other in the way that they can bind the target RNA (GUAGAG). The Ribonuclease A sequence was connected to the PPR complex through a flexible 6aa [GS]x linker.

Source

Synthetic

References