Coding

Part:BBa_K3657002:Design

Designed by: Jannik Berger   Group: iGEM20_Heidelberg   (2020-10-20)
Revision as of 23:50, 27 October 2020 by JuliaLanger (Talk | contribs) (→‎Design Notes)


RNA binding Pumilio Homology Domain (Uracil)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Pumilio Domains are specific for the different RNA bases, by varying in the aminoacid sequence in position 16 and 20. For the Uracil binding Pumby Domain, they are Asparagine and Glutamine, respectively. For more information on the design, visit iGEM Heidelberg 2020

Source

Nice Text

References