Coding

Part:BBa_K3610021:Design

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-04)
Revision as of 23:09, 27 October 2020 by Jtrott (Talk | contribs) (→‎Design Notes)


mCherry C-terminal - codon optimized for S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codons with a frequency lower than 10% were considered rare and were replaced for optimal expression. As alternative codons, the ones with the highest frequencies were chosen unless it added unwanted restriction sites and was therefore incompatible with iGEM standards.

The split was made at amino acids 158 and 159 from the full length mCherry.

Source

The sequence was taken from the registry and then adapted for expression in C. reinhardtii

References