Part:BBa_K3629015
Nourseothricin resistance expression construct with gibson homology sequences
Usage and Biology
This is the expression construct for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including Yarrowia lipolytica -the chassis for our project. Particularly for Y. lipolytica there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this antibiotic 50-200µg/mL is added to media depending on the organism.
The part is a key part of our collection and functions as a destination vector for other Y. lipolytica parts when cloned into a plasmid.
Design
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1590
Illegal EcoRI site found at 1449 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1449
Illegal NheI site found at 1584
Illegal SpeI site found at 1591
Illegal PstI site found at 1605
Illegal NotI site found at 7
Illegal NotI site found at 1598 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal EcoRI site found at 1449 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1591
Illegal EcoRI site found at 1449 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal EcoRI site found at 1449
Illegal XbaI site found at 16
Illegal SpeI site found at 1591
Illegal PstI site found at 1605 - 1000COMPATIBLE WITH RFC[1000]
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