Device

Part:BBa_K165096

Designed by: John Szymanski   Group: iGEM08_BrownTwo   (2008-10-29)
Revision as of 05:04, 30 October 2008 by Jszymans (Talk | contribs)

Zif268-HIV bs + MET25+ Gli1 repressor (CFPx2 tagged) on pRS304*

This construct is an example of the Tau element of our genetic limiter network. In this case, the pMET25 sets the threshold of limitation, allowing us to tune the threshold by varying the concentration of methionine in the media the cells are grown in. We constructed the Gli1 repressor in this devise with CFPx2 instead of mCherry, allowing us to distinguish it from the other repressors in the network. It is placed in a mutually inhibitory relationship with repressor alpha, in this case the Zif-HIV repressor. When the network is subthreshold, this tau repressor is expressed, and represses R, turning off its repression of the gene of interest. -

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2224
    Illegal XhoI site found at 1
    Illegal XhoI site found at 41
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 3990
    Illegal AgeI site found at 4740
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1395
    Illegal SapI.rc site found at 1988


[edit]
Categories
//chassis/eukaryote/yeast
//function/regulation/transcriptional
Parameters
None