Regulatory

Part:BBa_K3380109

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-25)
Revision as of 22:49, 27 October 2020 by Alexandru popov (Talk | contribs) (→‎Usage and Biology)


C. Metallidurans promoter with Zn transcription factor binding site

Fluorescent RNA aptamer construct flanked by F30 tRNA scaffolds to protect it from degradation. Its transcription is repressed by two component transcription factor (CzcS and CzcR). Upon addition of Zinc, the promoter allows the E.coli sigma 70 polymerase to transcribe the construct, resulting in green fluorescence if the DFHBI fluorophore is added.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None