Part:BBa_K3629011

Nourseothricin resistance gene

Nourseothricin acetyltransferase (nat1) gene from Streptomyces noursei.

Usage and Biology

This is the coding sequence for nourseothricin resistance, which is a robust selection marker that can be used in many eukaryotes, specifically yeast, including Yarrowia lipolytica -the chassis for our project. Particularly for Y. lipolytica there are not many available auxotrophy-based selection markers due to the limited number of auxotrophic strains publically available for use. Therefore, antibiotic resistance genes are a suitable alternative. To use this antibiotic 50-200µg/mL is added to media depending on the organism.

The part is a key part of our collection and functions as a destination vector for other Y. lipolytica parts when cloned into a plasmid with a promoter and terminator sequence attached.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Codon optimized for expression and function in Yarrowia lipolytica.