Part:BBa_K3588020

Synthetic Adhesin

This part is expressed on the extracellular membrane of E. Coli and from there has a an ability to adhere to an antigenic surface.

function

The part, when expressed correctly, allows for an increased ability for adherence to antigenic surfaces. The antigenic surface can be any common protein provided the synthetic adhesin VHH region is modified to be complimentary. In this case the VHH region has a complimentary binding site of GFP, and so if GFP is fixed to a surface, such as an ELISA plate then any bacteria expressing a complimentary binding region will be better able to adhere to given surface. This can be seen in the image included here but is better seen through our lab work results: https://2020.igem.org/Team:CLS_CLSG_UK/Results

design

This onstrict

File:T--CLS CLSG UK--SyntheticAdhesins.png

alt text

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 105
  Illegal PstI site found at 401
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 105
  Illegal PstI site found at 401
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  Illegal NotI site found at 2389
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 105
  Illegal BamHI site found at 1983
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 105
  Illegal PstI site found at 401
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 105
  Illegal PstI site found at 401
  Illegal PstI site found at 649
  Illegal PstI site found at 1096
  Illegal NgoMIV site found at 2004
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1244
  Illegal BsaI site found at 2063