Coding

Part:BBa_K3454038

Designed by: Ruijie Xiang   Group: iGEM20_ShanghaiTech_China   (2020-10-26)
Revision as of 21:45, 27 October 2020 by Fangzhiyu (Talk | contribs)


AsCas12a_R

This part is the template for AsCas12a_S542R extraction and purfication for reaction system.

Usage and Biology

Characterization

Result

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3855
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
    Illegal NgoMIV site found at 3399
    Illegal AgeI site found at 2296
    Illegal AgeI site found at 2767
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1039
    Illegal BsaI.rc site found at 2701
    Illegal SapI.rc site found at 289


[edit]
Categories
Parameters
None