Coding

Part:BBa_K3657024:Design

Designed by: Julia Langer   Group: iGEM20_Heidelberg   (2020-10-21)
Revision as of 21:32, 27 October 2020 by Liza ch (Talk | contribs) (→‎References)

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SoxS Trancription Activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The aminoacid sequence was obtained from the Uniprot database (P0A9E2). The nucletide sequence was then generated and codon optimised for E. coli.

Source

Escherichia coli

References

Wu J, Weiss B. Two-stage induction of the soxRS (superoxide response) regulon of Escherichia coli. J Bacteriol. 1992;174(12):3915-3920.

Dong C, Fontana J, Patel A, Carothers JM, Zalatan JG. Synthetic CRISPR-Cas gene activators for transcriptional reprogramming in bacteria [published correction appears in Nat Commun. 2018 Oct 15;9(1):4318]. Nat Commun. 2018;9(1):2489.

This part on Uniprot: https://www.uniprot.org/uniprot/P0A9E2