Composite

Part:BBa_K3654017

Designed by: Sin Young Park   Group: iGEM20_KSA_KOREA   (2020-10-27)
Revision as of 21:32, 27 October 2020 by Registry (Talk | contribs)

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PemrRAB-RBS-mScarlet-6His-rrnB T1-T7Te terminator PlacI-RBS-mprA-T7 terminator

This composite part is composed of a combination of two composite parts (BBa_K3654013 and BBa_K3654012). ㅁThis composite part is composed of a combination of two composite parts (BBa_K3654013 and BBa_K3654012). MprA expressed in BBa_K3654012 inhibits mScarlet expression by binding to the emrR promoter region of BBa_K3654013.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1378
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 130
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 159
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1378
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1378
    Illegal NgoMIV site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 650


[edit]
Categories
Parameters
None