Reporter

Part:BBa_K121012

Designed by: Masashi Kajita   Group: iGEM08_Tokyo_Tech   (2008-10-16)
Revision as of 03:54, 30 October 2008 by Ryo (Talk | contribs) (→‎Usage and Biology)

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Plac GFP on pSB6

IPTG inducible Plac (lacI regulated) + RBS + GFP + TT on pSB6 (Part:BBa_I739201).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 267
    Illegal BamHI site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 954

Usage and Biology

Identify Hill coefficient by IPTG induction

We identified hill coefficient of Plac_GFP in BBa_I739003 in E. Coli strain JM109.

figure. Transfer Function of BBa_K121012. The data points represent the mean of experimental measurements. The solid green curve was calculated by fitting a Hill function to the experimental measurements. The characteristics of Plac were calculated by fitting Hill function to the plots. We obtained the value of the hill coefficient. the value is 2.2. ⇒[http://2008.igem.org/Team:Tokyo_Tech/Protocol Protocol is here]





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