Composite

Part:BBa_K3396018

Designed by: Huiying Liu   Group: iGEM20_NUDT_CHINA   (2020-10-27)
Revision as of 21:20, 27 October 2020 by Liuhuiying (Talk | contribs)

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No part name specified with partinfo tag. CMV->Kozak->GFPnano->FKBP->FKBP->P2A ->Truncated TRIM21->FRB->BGH-pA

This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP. And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.

Usage and Biology

Our composite part BBa_K3396018 demonstrates how the Predator Pro system works. It regulates the target protein (EGFP) degradation under the control of external rapamycin signal.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]


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